Journal:

Article Title: GSK-3 promotes conditional association of CREB and its co-activators with MEIS1 to facilitate HOX-mediated transcription and oncogenesis

doi: 10.1016/j.ccr.2010.04.024

Figure Lengend Snippet: (A) Mouse myeloid progenitors immortalized by HOXA9 + MEIS1 were cultured in the presence or absence of 10 μM SB216763. Results show mean cell numbers expressed as fold-change compared to day 0 (error bars indicate ±SEM, n = 3).

Article Snippet: Transcriptional assay Transient transcriptional assays were performed in 293T cells as described previously ( Jacobs et al., 1999 ) using the Top-Flash reporter or reporter constructs under control of the HOXB1 ARE ( Jacobs et al., 1999 ), Gal-Meis1 ( Goh et al., 2009 ), HOXA9 promoter ( Wang et al., 2006 ), or FOS promoter (Addgene, plasmid 11983).

Techniques: Cell Culture

Journal:

Article Title: GSK-3 promotes conditional association of CREB and its co-activators with MEIS1 to facilitate HOX-mediated transcription and oncogenesis

doi: 10.1016/j.ccr.2010.04.024

Figure Lengend Snippet: (A) IP-western blot analysis was performed on 293T cells co-transduced with FLAG-CREB and HA-MEIS1, or AML cells transformed by HOXA9 + HA-MEIS1 in the presence or absence of 20 μM SB216763. Cell lysates were immunoprecipitated with anti-HA antibody conjugated beads, followed by western blot analysis using anti-FLAG (CREB), anti-HA (MEIS1), anti-CREB or anti-PBX1b antibodies.

Article Snippet: Transcriptional assay Transient transcriptional assays were performed in 293T cells as described previously ( Jacobs et al., 1999 ) using the Top-Flash reporter or reporter constructs under control of the HOXB1 ARE ( Jacobs et al., 1999 ), Gal-Meis1 ( Goh et al., 2009 ), HOXA9 promoter ( Wang et al., 2006 ), or FOS promoter (Addgene, plasmid 11983).

Techniques: Western Blot, Transduction, Transformation Assay, Immunoprecipitation